fsp27  (Novus Biologicals)

Journal: Molecular Metabolism

Article Title: TMEM135 deficiency improves hepatic steatosis by suppressing CD36 in a SIRT1-dependent manner

doi: 10.1016/j.molmet.2024.102080

Figure Lengend Snippet: Depletion of TMEM135 decreases lipid accumulation in the liver of HFD mice and FFA-treated primary hepatocytes and AML12 cell s. (A–C) WT and TMEM135KO mice were fed either NCD or HFD for 22 weeks (n = 6 per group). (A) Oil Red O staining of liver tissue section (Original magnification x100). (B) Immunoblots and densitometry for PLIN2, FSP27, and β-actin from the liver tissue of mice. Data represent mean ± SD, ∗∗p < 0.01, ∗p < 0.05, Two-way ANOVA. (C) Hepatic TG levels. Data represent mean ± SD, ∗∗p < 0.01, Two-way ANOVA. (D–F) Primary hepatocytes were isolated from the livers of WT and TMEM135KO mice and treated with FFA for 12 h. (D) Oil Red O staining and quantification of LD area (Original magnification x40, scale bar 20 μm). More than 100 cells per group in each experiment were measured. Data represent mean ± SD, ∗∗∗∗p < 0.0001, Two-way ANOVA. (E) Immunoblots and densitometry for PLIN2 and β-actin. Data represent mean ± SD, ∗∗∗∗p < 0.0001, ∗∗∗p < 0.001, Two-way ANOVA. (F) Quantification of TG levels. Data represent mean ± SD, ∗∗∗∗p < 0.0001, Two-way ANOVA. (G–I) sh-Scramble and sh-TMEM135 cells were treated with FFA for 12 h. (G) Oil Red O staining and quantification of LD area (Original magnification x100, scale bar 20 μm). More than 100 cells per group in each experiment were measured. Data represent mean ± SD, ∗∗∗∗p < 0.0001, ∗∗p < 0.01, Two-way ANOVA. (H) Immunoblots and densitometry for PLIN2 and β-actin. Data represent mean ± SD, ∗∗∗∗p < 0.0001, Two-way ANOVA. (I) Quantification of TG levels. Data represent mean ± SD, ∗∗∗∗p < 0.0001, ∗∗p < 0.01, Two-way ANOVA.

Article Snippet: CD36 antibody (#NB400-144) and FSP27 (#NB100-430) were bought from Novus Biological (Minneapolis, MN, USA).

Techniques: Staining, Western Blot, Isolation